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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Pepsin ELISA kit

E01P0699 Human Pepsin ELISA kit

The Human Pepsin ELISA kit can be used to identify samples from the human species. Pepsin can also be called PP, PEP.

Specifications of Human Pepsin ELISA kit

Product Information

Cat. No.

E01P0699

Product Name

Human Pepsin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PP ELISA kit uses an anti-PP antibody and an PP-HRP conjugate in a competitive enzyme immunoassay method. PP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PP antibody binding site between PP from samples and PP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PP. Since the number of sites is limited, as more sites are occupied by PP from the sample, fewer sites are left to bind PP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PP concentration in each sample is interpolated from this standard curve.


Quality Control on Human Pepsin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Pepsin ELISA kit

Summary of the Assay Procedure for Human Pepsin ELISA kit

Citations of Human Pepsin ELISA kit

E01P0699 has been referenced in the below publications:

Gold nanoclusters-based chemiluminescence resonance energy transfer method for sensitive and label-free detection of trypsin.

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