E11I0308 Human Dopamine ELISA kit
The Human Dopamine ELISA kit can be used to identify samples from the human species. Dopamine can also be called DA, 3,4-Dihydroxyphenethylamine, 3-Hydroxytyramine, Oxytyramine, Prolactin inhibiting factor, Prolactin inhibiting hormone, Intropin, Revivan.
Product Information | |
Cat. No. | E01D0043 |
Product Name | Human Dopamine ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/dL |
Sensitivity | 1.0 ng/dL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/dL | 1 vial |
STANDARD B (0.5mL) | 10 ng/dL | 1 vial |
STANDARD C (0.5mL) | 25 ng/dL | 1 vial |
STANDARD D (0.5mL) | 50 ng/dL | 1 vial |
STANDARD E (0.5mL) | 100 ng/dL | 1 vial |
STANDARD F (0.5mL) | 250 ng/dL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
DPA ELISA kit uses an anti-DPA antibody and an DPA-HRP conjugate in a competitive enzyme immunoassay method. DPA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-DPA antibody binding site between DPA from samples and DPA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of DPA. Since the number of sites is limited, as more sites are occupied by DPA from the sample, fewer sites are left to bind DPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DPA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between DPA and analogues was observed. | |
E01D0043 has been referenced in the below publications：
Exercise intervention can reduce the degree of drug dependence of patients with amphetamines/addiction by improving dopamine level and immunity and reducing negative emotions.
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