E01C0014 Human Collagen Type I ELISA kit
The Human Collagen Type I ELISA kit can be used to identify samples from the human species. Collagen Type I can also be called COL1, Collagen type I, Alpha 1 type I collagen, COL1A1, Collagen I alpha 1 polypeptide, Collagen Of Skin Tendon And Bone, Collagen Type 1, Collagen type I alpha 1, Pro alpha 1(I) collagen, Type I procollagen, Collagen alpha-1(II) chain, Collagen alpha-1(II) chain, Chondrocalcin, collagen alpha-1(I) chain preproprotein.
Product Information | |
Cat. No. | E01C0014 |
Product Name | Human Collagen Type I ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
COL1 ELISA kit uses an anti-COL1 antibody and an COL1-HRP conjugate in a competitive enzyme immunoassay method. COL1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-COL1 antibody binding site between COL1 from samples and COL1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of COL1. Since the number of sites is limited, as more sites are occupied by COL1 from the sample, fewer sites are left to bind COL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between COL1 and analogues was observed. | |
E01C0014 has been referenced in the below publications：
The CYP2E1 inhibitor DDC up-regulates MMP-1 expression in hepatic stellate cells via an ERK1/2- and Akt-dependent mechanism.
Combination treatment with asiaticoside and rapamycin: A new hope for in‑stent restenosis.
Role of up-regulation of THEM4 on collagen secretion in human renal tubular epithelial cells treated with high glucose.
Effect of THEM4 expression on collagen secretion in human embryo kidney epithelial cells treated with high glucose.
Photoluminescent biodegradable polyorganophosphazene: A promising scaffold material for in vivo application to promote bone regeneration.
In Vitro Release of Bioactive Bone Morphogenetic Proteins (GDF5, BB-1, and BMP-2) from a PLGA Fiber-Reinforced, Brushite-Forming Calcium Phosphate Cement.
Substituting plant vegetative parts with callus cell extracts: Case study with Woodfordia fruticosa Kurz. - A potent ingredient in skin care formulations.
SnoN residue (1-366) attenuates hypertrophic scars through resistance to transforming growth factor-β1-induced degradation.
Ultraviolet light-treated zirconia with different roughness affects function of human gingival fibroblasts in vitro: The potential surface modification developed from implant to abutment.
Profibrotic Effects of Endothelin-1 on Fibroblasts AreMediated by Aldosterone In Vitro: Relevance to the Pathogenesis and Therapy of Systemic Sclerosis and Pulmonary Arterial Hypertension.
Protective Effects of Triphala on Dermal Fibroblasts and Human Keratinocytes.
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