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  • bovine serum albumin elisa 1

BlueGene Biotech Human Hepcidin ELISA kit

E01H0051 Human Hepcidin ELISA kit

The Human Hepcidin ELISA kit can be used to identify samples from the human species. Hepcidin can also be called HAMP, HEPC, HFE2B, LEAP1, PLTR, hepcidin antimicrobial peptide.

Specifications of Human Hepcidin ELISA kit

Product Information

Cat. No.

E01H0051

Product Name

Human Hepcidin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Hepc ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Hepc. Standards or samples are then added to the microtiter plate wells and Hepc if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Hepc present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Hepc are added to each well to “sandwich” the Hepc immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Hepc and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Hepc concentration in each sample is interpolated from this standard curve.


Quality Control on Human Hepcidin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Hepc and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Hepcidin ELISA kit

Summary of the Assay Procedure for Human Hepcidin ELISA kit

Citations of Human Hepcidin ELISA kit

E01H0051 has been referenced in the below publications:

Study on the relationship between serum ferritin,Transferrin,hepcidin and type 2 diabetes.

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