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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Phospholipase A2, Secreted ELISA kit (E01S0173)

E01S0173 Human Phospholipase A2, Secreted ELISA kit

The Human Phospholipase A2, Secreted ELISA kit can be used to identify samples from the human species. Phospholipase A2, Secreted can also be called PLA2G2D, sPLA2S, s-PLA2, sPLA2, SPLASH, Secreted Phospholipase A2, Phosphatidylcholine 2-acylhydrolase 2D, Secretory-type PLA, stroma-associated homolog.

Products

Specifications of Human Phospholipase A2, Secreted ELISA kit

Product Information

Cat. No.

E01S0173

Product Name

Human Phospholipase A2, Secreted ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

100-2500 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

sPLA2 ELISA kit uses an anti-sPLA2 antibody and an sPLA2-HRP conjugate in a competitive enzyme immunoassay method. sPLA2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-sPLA2 antibody binding site between sPLA2 from samples and sPLA2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of sPLA2. Since the number of sites is limited, as more sites are occupied by sPLA2 from the sample, fewer sites are left to bind sPLA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sPLA2 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Phospholipase A2, Secreted ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between sPLA2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Phospholipase A2, Secreted ELISA kit

Summary of the Assay Procedure for Human Phospholipase A2, Secreted ELISA kit

Citations of Human Phospholipase A2, Secreted ELISA kit

E01S0173 has been referenced in the below publications:

Secretory phospholipase A2 and sOX40L levels and their correlations to severity of coronary lesion in acute coronary syndrome.

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