E01S0173 Human Phospholipase A2, Secreted ELISA kit
The Human Phospholipase A2, Secreted ELISA kit can be used to identify samples from the human species. Phospholipase A2, Secreted can also be called PLA2G2D, sPLA2S, s-PLA2, sPLA2, SPLASH, Secreted Phospholipase A2, Phosphatidylcholine 2-acylhydrolase 2D, Secretory-type PLA, stroma-associated homolog.
Product Information | |
Cat. No. | E01S0173 |
Product Name | Human Phospholipase A2, Secreted ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
| sPLA2 ELISA kit uses an anti-sPLA2 antibody and an sPLA2-HRP conjugate in a competitive enzyme immunoassay method. sPLA2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-sPLA2 antibody binding site between sPLA2 from samples and sPLA2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of sPLA2. Since the number of sites is limited, as more sites are occupied by sPLA2 from the sample, fewer sites are left to bind sPLA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sPLA2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between sPLA2 and analogues was observed. | |
E01S0173 has been referenced in the below publications：
Secretory phospholipase A2 and sOX40L levels and their correlations to severity of coronary lesion in acute coronary syndrome.
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