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  • bovine serum albumin elisa 1

BlueGene Biotech Human Asymmetric Dimethylaoyoinine ELISA kit (E01A0035)

E01A0035 Human Asymmetric Dimethylaoyoinine ELISA kit

Human Asymmetric Dimethylaoyoinine ELISA kit is suitable for the detection of samples from human species. Asymmetric Dimethylaoyoinine can also be called N-Dimethylarginine: NG, NG-Dimethylarginine dihydrochloride, Asymmetric Dimethylarginine, ADMA.

Products

Specifications of Human Asymmetric Dimethylaoyoinine ELISA kit

Product Information

Cat. No.

E01A0035

Product Name

Human Asymmetric Dimethylaoyoinine ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 μmoL/L

Sensitivity

0.1 μmoL/L

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C



Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 μmoL/L

1 vial

STANDARD B (0.5mL)

2.5 μmoL/L

1 vial

STANDARD C (0.5mL)

5.0 μmoL/L

1 vial

STANDARD D (0.5mL)

10 μmoL/L

1 vial

STANDARD E (0.5mL)

25 μmoL/L

1 vial

STANDARD F (0.5mL)

50 μmoL/L

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ADMA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ADMA. Standards or samples are then added to the microtiter plate wells and ADMA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADMA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADMA are added to each well to “sandwich” the ADMA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ADMA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADMA concentration in each sample is interpolated from this standard curve.



Quality Control on Human Asymmetric Dimethylaoyoinine ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-107%

Linearity

Diluent Ratio

Range %

1:2

95-102

1:4

90-107

1:8

93-109

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ADMA and analogues was observed.


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Summary of the Assay Procedure for Human Asymmetric Dimethylaoyoinine ELISA kit

Summary of the Assay Procedure for Human Asymmetric Dimethylaoyoinine ELISA kit

Citations of Human Asymmetric Dimethylaoyoinine ELISA kit

E01A0035 has been referenced in the below publications:

Dickkopf1 destabilizes atherosclerotic plaques and promotes plaque formation by inducing apoptosis of endothelial cells through activation of ER stress.

Brain Volumetrics, Regional Cortical Thickness and Radiographic Findings in Adults with Cyanotic Congenital Heart Disease.

Effect of super-flux polyethersulfone dialysis membrane on the removal of asymmetrical dimethylarginine: a pilot clinical study.

The Effect and Mechanism of Asymmetric Dimethylarginine Regulating Trophoblastic Autophagy on Fetal Growth Restriction.

The role and mechanism of asymmetric dimethylarginine in fetal growth restriction via interference with endothelial function and angiogenesis.

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