E01P0750 Human Peroxiredoxin 2 ELISA kit
The Human Peroxiredoxin 2 ELISA kit can be used to identify samples from the human species. Peroxiredoxin 2 can also be called PRP, NKEFB, PRXII, TDPX1, TSA, Thioredoxin-Dependent Peroxide Reductase 1, Thiol-Specific Antioxidant 1, Natural Killer-Enhancing Factor B, Thioredoxin Peroxidase 1, Torin.
Product Information | |
Cat. No. | E01P0750 |
Product Name | Human Peroxiredoxin 2 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PRDX2 ELISA kit uses an anti-PRDX2 antibody and an PRDX2-HRP conjugate in a competitive enzyme immunoassay method. PRDX2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PRDX2 antibody binding site between PRDX2 from samples and PRDX2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PRDX2. Since the number of sites is limited, as more sites are occupied by PRDX2 from the sample, fewer sites are left to bind PRDX2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRDX2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PRDX2 and analogues was observed. | |
E01P0750 has been referenced in the below publications：
Proteomics and Peptidomics Analysis of Lung Adenocarcinoma Malignant Pleural Effusion.
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