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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Plasminogen Activator Inhibitor 1 ELISA kit (E01P0028)

E01P0028 Human Plasminogen Activator Inhibitor 1 ELISA kit

The Human Plasminogen Activator Inhibitor 1 ELISA kit can be used to identify samples from the human species. Plasminogen Activator Inhibitor 1 can also be called PAI 1, SERPINE1, PLANH1, Serpin Peptidase Inhibitor Clade E Member 1, Nexin,Plasminogen Activator Inhibitor Type 1 Serpin E1, Endothelial plasminogen activator inhibitor.

Products

Specifications of Human Plasminogen Activator Inhibitor 1 ELISA kit

Product Information

Cat. No.

E01P0028

Product Name

Human Plasminogen Activator Inhibitor 1 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PAI 1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PAI 1. Standards or samples are then added to the microtiter plate wells and PAI 1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PAI 1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PAI 1 are added to each well to “sandwich” the PAI 1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound coPAI 1nents. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PAI 1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAI 1 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Plasminogen Activator Inhibitor 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PAI 1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Plasminogen Activator Inhibitor 1 ELISA kit

Summary of the Assay Procedure for Human Plasminogen Activator Inhibitor 1 ELISA kit

Citations of Human Plasminogen Activator Inhibitor 1 ELISA kit

E01P0028 has been referenced in the below publications:

Effects of Chushi Huayu Prescription on Endothelin-1(ET-1) and Plasminogen Activator Inhibitor-1 (PAI-1) of Hyperuricemia Patients.

RESEARCH OF SUBCLINICAL ATHEROSCLEROSIS IN TYPE 2 DIABETIC PATIENTS WITH METABOLIC SYNDROME.

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