E01A0015 Human Active Caspase 3 ELISA kit
Human Active caspase 3 ELISA kit is suitable for the detection of samples from human species. Active caspase 3 can also be called CPP32, CPP32B, SCA1, Apoptain, Yama, Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 3, SREBP cleavage activity 1, Active casp3, casp3, casp-3, casp 3.
Product Information | |
Cat. No. | E01A0015 |
Product Name | Human Active caspase 3 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Active caspase 3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Active caspase 3 antibody and an Active caspase 3-HRP conjugate. The assay sample and buffer are incubated together with Active caspase 3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Active caspase 3 concentration since Active caspase 3 from samples and Active caspase 3-HRP conjugate compete for the anti-Active caspase 3 antibody binding site. Since the number of sites is limited, as more sites are occupied by Active caspase 3 from the sample, fewer sites are left to bind Active caspase 3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Active caspase 3 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 92-109% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-103 | |
1:4 | 91-109 | |
1:8 | 89-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between active casp3 and analogues was observed. | |
E01A0015 has been referenced in the below publications:
Caspases as prognostic markers and mortality predictors in acute organophosphorus poisoning.
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