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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human D Dimer ELISA kit (E01D0224)

E01D0224 Human D Dimer ELISA kit

The Human D Dimer ELISA kit can be used to identify samples from the human species. D Dimer can also be called D2D, D 2 Dimer, D-Dimer.

Products

Specifications of Human D Dimer ELISA kit

Product Information

Cat. No.

E01D0224

Product Name

Human D Dimer ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

D2D ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for D2D. Standards or samples are then added to the microtiter plate wells and D2D if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of D2D present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for D2D are added to each well to “sandwich” the D2D immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound coD2Dnents. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain D2D and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The D2D concentration in each sample is interpolated from this standard curve.


Quality Control on Human D Dimer ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between D2D and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human D Dimer ELISA kit

Summary of the Assay Procedure for Human D Dimer ELISA kit

Citations of Human D Dimer ELISA kit

E01D0224 has been referenced in the below publications:

Study the role of vWF in assessing the Prethrombotic state and the severity of COPD in COPD patients.

The detection and significance of serum ICAM-1 and  D-D in the patients with chronic severe hepatitis B and the studies of the influence on both with entecavir.

Type II Minimal-Invasive Extracorporeal Circuit for Aortic Valve Replacement: A Randomized Controlled Trial.

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