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BlueGene Biotech Human Endothelial Selectin ELISA kit (E01S0079)

E01S0079 Human Endothelial Selectin ELISA kit

The Human Endothelial Selectin ELISA kit can be used to identify samples from the human species. Endothelial Selectin can also be called E selectin, CD62E, CD62-E, E-Selectin, ELAM, ELAM1, ESEL, SEL-E, E-LAM, E-LAM1, E-SEL, LECAM2, Endothelial Leukocyte Adhesion Molecule 1, CD62 Antigen-Like Family Member E.

Products

Specifications of Human Endothelial Selectin ELISA kit

Product Information

Cat. No.

E01S0079

Product Name

Human Endothelial Selectin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

25-500 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

25 pg/mL

1 vial

STANDARD C (0.5mL)

50 pg/mL

1 vial

STANDARD D (0.5mL)

100 pg/mL

1 vial

STANDARD E (0.5mL)

250 pg/mL

1 vial

STANDARD F (0.5mL)

500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

E selectin ELISA kit uses an anti-E selectin antibody and an E selectin-HRP conjugate in a competitive enzyme immunoassay method. E selectin-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-E selectin antibody binding site between E selectin from samples and E selectin-HRP conjugate, the intensity of the color is inversely proportional to the concentration of E selectin. Since the number of sites is limited, as more sites are occupied by E selectin from the sample, fewer sites are left to bind E selectin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The E selectin concentration in each sample is interpolated from this standard curve.


Quality Control on Human Endothelial Selectin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between E selectin and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Endothelial Selectin ELISA kit

Summary of the Assay Procedure for Human Endothelial Selectin ELISA kit

Citations of Human Endothelial Selectin ELISA kit

E01S0079 has been referenced in the below publications:

Research on the relationship between MMP-2, MCP-1, E-seletin, Aopps and p38MAPK signaling pathway in elderly patients with depression.

Effect of soybean isoflavones on the metabolism and anti-oxidative stress in MG-63 cells.

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