E01C0663 Human Complement fragment 5a ELISA kit
The Human Complement fragment 5a ELISA kit can be used to identify samples from the Human species. Complement fragment 5a can also be called C5a anaphylatoxin,Complement C5 alpha' chain.
Specifications of Human Complement fragment 5a ELISA kit
Product Information | |
Cat. No. | E01C0663 |
Product Name | Human Complement fragment 5a ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
C5a ELISA kit uses an anti-C5a antibody and an C5a-HRP conjugate in a competitive enzyme immunoassay method. C5a-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-C5a antibody binding site between C5a from samples and C5a-HRP conjugate, the intensity of the color is inversely proportional to the concentration of C5a. Since the number of sites is limited, as more sites are occupied by C5a from the sample, fewer sites are left to bind C5a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C5a concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Complement fragment 5a ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between C5a and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Complement fragment 5a ELISA kit
Citations of Human Complement fragment 5a ELISA kit
E01C0663 has been referenced in the below publications：
Doxorubicin Delivered Using Nanoparticles Camouflaged with Mesenchymal Stem Cell Membranes to Treat Colon Cancer.
Mild hypothermia inhibits systemic and cerebral complement activation in a swine model of cardiac arrest.
