E01G0034 Human Alpha Galactosyl ELISA kit
The Human Alpha Galactosyl ELISA kit can be used to identify samples from the human species. Alpha Galactosyl can also be called Alpha Gal, Galactose-alpha-1,3-galactose, alpha gal, GLA, α-D-galactoside galactohydrolase, α-GAL, α-GAL A, GLA, GALA, galactosidase alpha, Alpha-D-galactosidase A, Alpha-D-galactoside galactohydrolase.
Product Information | |
Cat. No. | E01G0034 |
Product Name | Human Alpha Galactosyl ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Alpha Gal ELISA kit uses an anti-Alpha Gal antibody and an Alpha Gal-HRP conjugate in a competitive enzyme immunoassay method. Alpha Gal-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Alpha Gal antibody binding site between Alpha Gal from samples and Alpha Gal-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Alpha Gal. Since the number of sites is limited, as more sites are occupied by Alpha Gal from the sample, fewer sites are left to bind Alpha Gal-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Alpha Gal concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Alpha Gal and analogues was observed. | |
E01G0034 has been referenced in the below publications：
Improved the biocompatibility of cancellous bone with compound physicochemical decellularization process.
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