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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Secretoglobin, Family 1A, Member 1 ELISA Kit (E01U0019)

E01U0019 Human Secretoglobin, Family 1A, Member 1 ELISA Kit

The Human Secretoglobin, Family 1A, Member 1 ELISA Kit can be used to identify samples from the human species. Secretoglobin, Family 1A, Member 1 can also be called SCGB1A1, CC10, CC16, CCPBP, CCSP, UGB, UP-1, UP1, secretoglobin family 1A member 1.

Products

Specifications of Human Secretoglobin, Family 1A, Member 1 ELISA Kit

Product Information

Cat. No.

E01U0019

Product Name

Human Secretoglobin, Family 1A, Member 1 ELISA Kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1ng/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SCGB1A1/CC16 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SCGB1A1/CC16. Standards or samples are then added to the microtiter plate wells and SCGB1A1/CC16 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SCGB1A1/CC16 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SCGB1A1/CC16 are added to each well to “sandwich” the SCGB1A1/CC16 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SCGB1A1/CC16 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCGB1A1/CC16 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Secretoglobin, Family 1A, Member 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SCGB1A1/CC16 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Secretoglobin, Family 1A, Member 1 ELISA Kit

Summary of the Assay Procedure for Human Secretoglobin, Family 1A, Member 1 ELISA Kit

Citations of Human Secretoglobin, Family 1A, Member 1 ELISA Kit

E01U0019 has been referenced in the below publications:

Correlation of serum KL-6 and CC16 levels with neurodevelopmental outcome in premature infants at 12 months corrected age.

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