E01U0019 Human Secretoglobin, Family 1A, Member 1 ELISA Kit
The Human Secretoglobin, Family 1A, Member 1 ELISA Kit can be used to identify samples from the human species. Secretoglobin, Family 1A, Member 1 can also be called SCGB1A1, CC10, CC16, CCPBP, CCSP, UGB, UP-1, UP1, secretoglobin family 1A member 1.
Product Information | |
Cat. No. | E01U0019 |
Product Name | Human Secretoglobin, Family 1A, Member 1 ELISA Kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/mL |
Sensitivity | 0.1ng/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SCGB1A1/CC16 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SCGB1A1/CC16. Standards or samples are then added to the microtiter plate wells and SCGB1A1/CC16 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SCGB1A1/CC16 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SCGB1A1/CC16 are added to each well to “sandwich” the SCGB1A1/CC16 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SCGB1A1/CC16 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCGB1A1/CC16 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SCGB1A1/CC16 and analogues was observed. | |
E01U0019 has been referenced in the below publications：
Correlation of serum KL-6 and CC16 levels with neurodevelopmental outcome in premature infants at 12 months corrected age.
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