E01A0863 Human Aquaporin 1 ELISA kit
The Human Aquaporin 1 ELISA kit can be used to identify samples from the human species. Aquaporin 1 can also be called AQP-CHIP; CHIP28.
Human Aquaporin 1 ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
AQP ELISA kit uses an anti-AQP antibody and an AQP-HRP conjugate in a competitive enzyme immunoassay method. AQP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-AQP antibody binding site between AQP from samples and AQP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of AQP. Since the number of sites is limited, as more sites are occupied by AQP from the sample, fewer sites are left to bind AQP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AQP concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between AQP1 and analogues was observed.
E01A0863 has been referenced in the below publications：
Expression and Significance of Aquaporin-1 in Serum and Peritoneal Fluid from Women with Endometriosis.
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