E01T0568 Human Tumor Necrosis Factor Receptor 2 ELISA kit
The Human Tumor Necrosis Factor Receptor 2 ELISA kit can be used to identify samples from the human species. Tumor Necrosis Factor Receptor 2 can also be called TNFRSF1B, CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, p75, p75TNFR, tumor necrosis factor receptor superfamily member 1B, TNF receptor superfamily member 1B.
Product Information | |
Cat. No. | E01T0568 |
Product Name | Human Tumor Necrosis Factor Receptor 2 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 250 pg/ml | 1 vial |
STANDARD C (0.5mL) | 500 pg/ml | 1 vial |
STANDARD D (0.5mL) | 1000 pg/ml | 1 vial |
STANDARD E (0.5mL) | 2500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 5000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TNFR2 ELISA kit uses an anti-TNFR2 antibody and an TNFR2-HRP conjugate in a competitive enzyme immunoassay method. TNFR2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TNFR2 antibody binding site between TNFR2 from samples and TNFR2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TNFR2. Since the number of sites is limited, as more sites are occupied by TNFR2 from the sample, fewer sites are left to bind TNFR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNFR2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TNFR2 and analogues was observed. | |
E01T0568 has been referenced in the below publications：
The Significance of the Assays of Serum sIL-2R and sTNFRII in the Research of Sarcoidosis.
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