Human Total Adiponectin ELISA kit - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human Total Adiponectin ELISA kit

E01A0125 Human Total Adiponectin ELISA kit

The Human Total Adiponectin ELISA kit can be used to identify samples from the human species. Total Adiponectin can also be called 30 kDa adipocyte complement related protein, 30 kDa adipocyte complement-related protein, ACDC, ACRP 30, ACRP30, ADIPO, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, Adiponectin, AdipoQ, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, ADIPQTL1, ADPN, APM 1, apM-1, ApM1, C1q and collagen domain-containing protein, GBP 28, GBP28, Gelatin binding protein, Gelatin binding protein 28, Gelatin-binding protein 2.

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Specifications of Human Total Adiponectin ELISA kit

Product Information
Cat. No.	E01A0125
Product Name	Human Total Adiponectin ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	2.5-50 µg/mL
Sensitivity	0.1 ug/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 µg/mL	1 vial
STANDARD B (0.5mL)	2.5 µg/mL	1 vial
STANDARD C (0.5mL)	5.0 µg/mL	1 vial
STANDARD D (0.5mL)	10 µg/mL	1 vial
STANDARD E (0.5mL)	25 µg/mL	1 vial
STANDARD F (0.5mL)	50 µg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

ADPN ELISA kit uses an anti-ADPN antibody and an ADPN-HRP conjugate in a competitive enzyme immunoassay method. ADPN-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ADPN antibody binding site between ADPN from samples and ADPN-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ADPN. Since the number of sites is limited, as more sites are occupied by ADPN from the sample, fewer sites are left to bind ADPN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADPN concentration in each sample is interpolated from this standard curve.

Quality Control on Human Total Adiponectin ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between ADPN and analogues was observed.

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Summary of the Assay Procedure for Human Total Adiponectin ELISA kit

Citations of Human Total Adiponectin ELISA kit

E01A0125 has been referenced in the below publications：

Change of serum levels of adiponectin and YKL-40 in patients witll different coronary heart diseases and its correlation with the severity of coronary artery lesion.

PLASMA ADIPONECTIN LEVELS AND ITS CORRELATION STUDY WITH BRAIN NATRIURETIC PEPTIDE, TUMOR NECROSIS FACTOR-a AND INSULIN RESISTANCE IN PATIENTS WITH CHRONIC HEART FAILURE.

Study on the serum adiponectin concentration in neonatal septicemia and the relationship among serum adiponectin, blood fat and blood sugar.

A reciprocal inhibitory relationship between adiponectin and mammalian cytosolic thioredoxin.