E01L0021 Human Ornithine carbamoyltransferase, mitochondrial ELISA kit
The Human Ornithine carbamoyltransferase, mitochondrial ELISA kit can be used to identify samples from the human species. Ornithine carbamoyltransferase, mitochondrial can also be called OCTD, OCT, OTCase, Ornithine Transcarbamylase, Ornithine Carbamoyltransferase.
Product Information | |
Cat. No. | E01O0739 |
Product Name | Human Ornithine carbamoyltransferase, mitochondrial ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 mU/mL |
Sensitivity | 1.0 mU/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mU/mL | 1 vial |
STANDARD B (0.5mL) | 10 mU/mL | 1 vial |
STANDARD C (0.5mL) | 25 mU/mL | 1 vial |
STANDARD D (0.5mL) | 50 mU/mL | 1 vial |
STANDARD E (0.5mL) | 100 mU/mL | 1 vial |
STANDARD F (0.5mL) | 250 mU/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
OTC ELISA kit uses an anti-OTC antibody and an OTC-HRP conjugate in a competitive enzyme immunoassay method. OTC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-OTC antibody binding site between OTC from samples and OTC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of OTC. Since the number of sites is limited, as more sites are occupied by OTC from the sample, fewer sites are left to bind OTC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OTC concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between OTC and analogues was observed. | |
E01O0739 has not been referenced.
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