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E01A0039 Human Total β Amyloid Protein ELISA Kit

Human Total β Amyloid Protein ELISA Kit is suitable for the detection of samples from human species.

Specifications of E01A0039 Human Aβ ELISA Kit

Porduct's Information

Cat.NO.

E01A0039

Porduct's Name

Human Alkaline Phosphatase ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100ng/mL

Sensitivity

1.0 ng/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AΒ ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AΒ. Standards or samples are then added to the microtiter plate wells and AΒ if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AΒ present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AΒ are added to each well to “sandwich” the AΒ immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AΒ and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AΒ concentration in each sample is interpolated from this standard curve.a


Quality Control On Human AΒ ELISA Kit

CV

Intra Variation% <10%

Inter Variation% <12%

Recovery%

95-105

Linearity

Diluent ratio

Range %

1:2

92-102

1:4

91-107

1:8

91-105

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AΒ and analogues was observed.


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Summary of The Assay Procedures For E01A0039 Human AΒ ELISA Kit

Summary of The Assay Procedures For E01A0039 Human AΒ ELISA Kit

Citations of Human Total β Amyloid Protein ELISA Kit

The study of the Characteristics of Cognitive Impairment after Acute Mild-to-Moderate Ischemic Stroke and the Role of SAA and Aβ in Cognitive Impairment

Product: E01S0228 Human Serum amyloid A ELISA kit; E01M0002 Human α2-Macroglobulin ELISA;

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