Human Total β Amyloid Protein ELISA Kit is suitable for the detection of samples from human species.
Specifications of E01A0039 Human Aβ ELISA Kit
Porduct's Information | |
Cat.NO. | E01A0039 |
Porduct's Name | Human Alkaline Phosphatase ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AΒ ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AΒ. Standards or samples are then added to the microtiter plate wells and AΒ if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AΒ present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AΒ are added to each well to “sandwich” the AΒ immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AΒ and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AΒ concentration in each sample is interpolated from this standard curve.a |
Quality Control On Human AΒ ELISA Kit
CV | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery% | 95-105 | |
Linearity | Diluent ratio | Range % |
1:2 | 92-102 | |
1:4 | 91-107 | |
1:8 | 91-105 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AΒ and analogues was observed. | |
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Summary of The Assay Procedures For E01A0039 Human AΒ ELISA Kit
Citations of Human Total β Amyloid Protein ELISA Kit
The study of the Characteristics of Cognitive Impairment after Acute Mild-to-Moderate Ischemic Stroke and the Role of SAA and Aβ in Cognitive Impairment
Product: E01S0228 Human Serum amyloid A ELISA kit; E01M0002 Human α2-Macroglobulin ELISA;
