E01C0876 Human sCD14 ELISA Kit
The Human sCD14 ELISA kit can be used to identify samples from the human species. sCD14 can also be called sentrez:929, SCD14 molecule.
Specifications of Human sCD14 ELISA kit
Product Information | |
Cat. No. | E01C0876 |
Product Name | Human sCD14 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/mL |
Sensitivity | 0.1ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
sCD14 ELISA kit uses an anti-sCD14 antibody and an sCD14-HRP conjugate in a competitive enzyme immunoassay method. sCD14-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-sCD14 antibody binding site between sCD14 from samples and sCD14-HRP conjugate, the intensity of the color is inversely proportional to the concentration of sCD14. Since the number of sites is limited, as more sites are occupied by sCD14 from the sample, fewer sites are left to bind sCD14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sCD14 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human sCD14 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between sCD14 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human sCD14 ELISA kit
Citations of Human sCD14 ELISA kit
E01C0876 has been referenced in the below publications：
Markers of the innate immune system in the cerebrospinal fluid in patients with severe depression.
