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E01A0044 Human Adenosine Diphosphate ELISA Kit

Human Adenosine diphosphate ELISA kit is suitable for the detection of samples from human species.

Specifications of E01A0044 Human Adenosine Diphosphate ELISA Kit

Porduct's Information

Cat.NO.

E01A0044

Porduct's Name

Human Adenosine diphosphate ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ADP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADP antibody and an ADP-HRP conjugate. The assay sample and buffer are incubated together with ADP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADP concentration since ADP from samples and ADP-HRP conjugate compete for the anti-ADP antibody binding site. Since the number of sites is limited, as more sites are occupied by ADP from the sample, fewer sites are left to bind ADP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADP concentration in each sample is interpolated from this standard curve.


Quality Control On Human ADP ELISA Kit

CV

Intra Variation% <10%

Inter Variation% <12%

Recovery%

93-103

Linearity

Diluent ratio

Range %

1:2

92-104

1:4

90-108

1:8

89-113

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ADP and analogues was observed.


BlueGene Biotech Product Show

Summary of The Assay Procedures For E01A0044 Human ADP ELISA Kit

Summary of The Assay Procedures For E01A0044 Human ADP ELISA Kit

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