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BlueGene Biotech Human Superoxide Dismutase ELISA kit

E01S0012 Human Superoxide Dismutase ELISA kit

The Human Superoxide Dismutase ELISA kit can be used to identify samples from the human species. Superoxide Dismutase can also be called SOD, SOD1, Cu-Zn SOD, Ipo1, IPOA, cytosolic, SOD, Soluble, ALS, ALS1, amyotrophic lateral sclerosis 1, adult, Cu, Zn superoxide dismutase, hSod1, indophenoloxidase A, Superoxide dismutase 1, superoxide dismutase 1, soluble, superoxide dismutase, cystolic.

Specifications of Human Superoxide Dismutase ELISA kit

Product Information

Cat. No.

E01S0012

Product Name

Human Superoxide Dismutase ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ug/ml

Sensitivity

0.1 ug/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

1.0 ug/mL

1 vial

STANDARD C (0.5mL)

2.5 ug/mL

1 vial

STANDARD D (0.5mL)

5.0 ug/mL

1 vial

STANDARD E (0.5mL)

10 ug/mL

1 vial

STANDARD F (0.5mL)

25 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SOD ELISA kit uses an anti-SOD antibody and an SOD-HRP conjugate in a competitive enzyme immunoassay method. SOD-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SOD antibody binding site between SOD from samples and SOD-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SOD. Since the number of sites is limited, as more sites are occupied by SOD from the sample, fewer sites are left to bind SOD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SOD concentration in each sample is interpolated from this standard curve.


Quality Control on Human Superoxide Dismutase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SOD and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Superoxide Dismutase ELISA kit

Summary of the Assay Procedure for Human Superoxide Dismutase ELISA kit

Citations of Human Superoxide Dismutase ELISA kit

E01S0012 has been referenced in the below publications:

The Clinical Study on the effect of Kun Fu Kang Capsule on Red Cell Immunology with Chronic Pelvic Inflammatory Disease.

Analysis of Cells Types and Protein of Bronchoalveolar Lavage Fluid of Coal Worker's Pneumoconiosis.

阻塞性睡眠呼吸暂停低通气综合征患者线粒体氧化唱抗氧化功能的变化。

A cell model of nonalcoholic steatohepatitis induced by FFA: establishment and dynamic monitoring.

The Effects of Mouse Nerve Growth Factor on Hypoxic-ischemic Encephalopathy and the Neuroprotective Effect in Neonates.

Antioxidant Blueberry Anthocyanins Induce Vasodilation via PI3K/Akt Signaling Pathway in High-Glucose-Induced Human Umbilical Vein Endothelial Cells.

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