E01L0018 Human Laminin ELISA kit
The Human Laminin ELISA kit can be used to identify samples from the human species. Laminin can also be called LN, LAM.
Human Laminin ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
LN ELISA kit uses an anti-LN antibody and an LN-HRP conjugate in a competitive enzyme immunoassay method. LN-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LN antibody binding site between LN from samples and LN-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LN. Since the number of sites is limited, as more sites are occupied by LN from the sample, fewer sites are left to bind LN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LN concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between LN and analogues was observed.
E01L0018 has been referenced in the below publications：
Flavonoid-rich Scabiosa comosa inflorescence extract attenuates CCl4-induced hepatic fibrosis by modulating TGF-β-induced Smad3 phosphorylation.
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