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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Complement component 3b ELISA kit

E01C0113 Human Complement component 3b ELISA kit

The Human Complement component 3b ELISA kit can be used to identify samples from the human species. Complement component 3b can also be called Complement fragment 3b, ASPa, C3b, CPAMD1, HEL-S-62p.

Specifications of Human Complement component 3b ELISA kit

Product Information

Cat. No.

E01C0113

Product Name

Human Complement component 3b ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1ng/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

C3b ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for C3b. Standards or samples are then added to the microtiter plate wells and C3b if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of C3b present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for C3b are added to each well to “sandwich” the C3b immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain C3b and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C3b concentration in each sample is interpolated from this standard curve.


Quality Control on Human Complement component 3b ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between C3b and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Complement component 3b ELISA kit

Summary of the Assay Procedure for Human Complement component 3b ELISA kit

Citations of Human Complement component 3b ELISA kit

E01C0113 has been referenced in the below publications:

Mild hypothermia inhibits systemic and cerebral complement activation in a swine model of cardiac arrest.


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