E01T0143 Human Total Bilirubin ELISA kit
The Human Total Bilirubin ELISA kit can be used to identify samples from the human species. Total Bilirubin can also be called BR.
Human Total Bilirubin ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
|100 nmol/ml||1 vial|
WASH SOLUTION (100 x)
Principle of the Assay
TBIL ELISA kit uses an anti-TBIL antibody and an TBIL-HRP conjugate in a competitive enzyme immunoassay method. TBIL-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TBIL antibody binding site between TBIL from samples and TBIL-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TBIL. Since the number of sites is limited, as more sites are occupied by TBIL from the sample, fewer sites are left to bind TBIL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TBIL concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between TBIL and analogues was observed.
E01T0143 has been referenced in the below publications：
Mild hypothermia inhibits systemic and cerebral complement activation in a swine model of cardiac arrest.
Related Bluegene Biotech Products
BlueGene Biotech News