E01T0143 Human Total Bilirubin ELISA kit
The Human Total Bilirubin ELISA kit can be used to identify samples from the human species. Total Bilirubin can also be called BR.
Specifications of Human Total Bilirubin ELISA kit
Product Information | |
Cat. No. | E01T0143 |
Product Name | Human Total Bilirubin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 nmol/ml |
Sensitivity | 1.0 nmol/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 nmol/ml | 1 vial |
STANDARD B (0.5mL) | 5.0 nmol/ml | 1 vial |
STANDARD C (0.5mL) | 10 nmol/ml | 1 vial |
STANDARD D (0.5mL) | 25 nmol/ml | 1 vial |
STANDARD E (0.5mL) | 50 nmol/ml | 1 vial |
STANDARD F (0.5mL) | 100 nmol/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TBIL ELISA kit uses an anti-TBIL antibody and an TBIL-HRP conjugate in a competitive enzyme immunoassay method. TBIL-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TBIL antibody binding site between TBIL from samples and TBIL-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TBIL. Since the number of sites is limited, as more sites are occupied by TBIL from the sample, fewer sites are left to bind TBIL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TBIL concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Total Bilirubin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TBIL and analogues was observed. | |
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Summary of the Assay Procedure for Human Total Bilirubin ELISA kit
Citations of Human Total Bilirubin ELISA kit
E01T0143 has been referenced in the below publications：
Mild hypothermia inhibits systemic and cerebral complement activation in a swine model of cardiac arrest.
