E01A0832 Human Advanced oxidation protein products ELISA kit
The Human Advanced oxidation protein products ELISA kit can be used to identify samples from the Human species. Advanced oxidation protein products can also be called AOPP.
Specifications of Human Advanced oxidation protein products ELISA kit
Product Information | |
Cat. No. | E01A0832 |
Product Name | Human Advanced oxidation protein products ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AOPP ELISA kit uses an anti-AOPP antibody and an AOPP-HRP conjugate in a competitive enzyme immunoassay method. AOPP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-AOPP antibody binding site between AOPP from samples and AOPP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of AOPP. Since the number of sites is limited, as more sites are occupied by AOPP from the sample, fewer sites are left to bind AOPP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AOPP concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Advanced oxidation protein products ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AOPP and analogues was observed. | |
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Summary of the Assay Procedure for Human Advanced oxidation protein products ELISA kit
Citations of Human Advanced oxidation protein products ELISA kit
E01V0023 has been referenced in the below publications：
Effects of recombinant human growth hormone on the expression of CD47, L-selectin and advanced oxidation protein products in elderly patients with multiple organ dysfunction syndrome.
Research on the relationship between MMP-2, MCP-1, E-seletin, Aopps and p38MAPK signaling pathway in elderly patients with depression.
Comprehensive analysis of oxidative stress indexs in hunman serum with endemic fluorosis of coal burning.
