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BlueGene Biotech Human High affinity IgE Receptor Alpha /β Chain ELISA kit

E01H0002 Human High affinity IgE Receptor Alpha /β Chain ELISA kit

The Human High affinity IgE Receptor Alpha /β Chain ELISA kitcan be used to identify samples from the human species. High affinity IgE Receptor Alpha /β Chain can also be called FcεRI Alpha/beta, Fc epsilon RI Alpha/beta.

Specifications of Human High affinity IgE Receptor Alpha /β Chain ELISA kit

Product Information

Cat. No.

E01H0002

Product Name

Human High affinity IgE Receptor Alpha /β Chain ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 µg/mL

Sensitivity

0.1 ug/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 µg/mL

1 vial

STANDARD B (0.5mL)

2.5 µg/mL

1 vial

STANDARD C (0.5mL)

5.0 µg/mL

1 vial

STANDARD D (0.5mL)

10 µg/mL

1 vial

STANDARD E (0.5mL)

25 µg/mL

1 vial

STANDARD F (0.5mL)

50 µg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

FcεRIAlpha /β ELISA kit uses an anti-FcεRIAlpha /β antibody and an FcεRIAlpha /β-HRP conjugate in a competitive enzyme immunoassay method. FcεRIAlpha /β-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-FcεRIAlpha /β antibody binding site between FcεRIAlpha /β from samples and FcεRIAlpha /β-HRP conjugate, the intensity of the color is inversely proportional to the concentration of FcεRIAlpha /β. Since the number of sites is limited, as more sites are occupied by FcεRIAlpha /β from the sample, fewer sites are left to bind FcεRIAlpha /β-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FcεRIAlpha /β concentration in each sample is interpolated from this standard curve.


Quality Control on Human High affinity IgE Receptor Alpha /β Chain ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between FcεRIAlpha /β and analogues was observed.


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Summary of the Assay Procedure for Human High affinity IgE Receptor Alpha /β Chain ELISA kit

Summary of the Assay Procedure for Human High affinity IgE Receptor Alpha /β Chain ELISA kit

Citations of Human High affinity IgE Receptor Alpha /β Chain ELISA kit

E01H0002 has been referenced in the below publications:

THE ASSOCIATION ANALYSIS OF ANTI-IGE ANTIBODY AND ANTI-FCεRI ANTIBODY WITH AUTOIMMUNE LIVER DISEASE.

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