E01K0079 Human Kallikrein 5 ELISA kit
The Human Kallikrein 5 ELISA kit can be used to identify samples from the human species. Kallikrein 5 can also be called KLK-L2, KLKL2, SCTE, Kallikrein-Related Peptidase 5, Kallikrein-like protein 2, Stratum corneum tryptic enzyme.
Product Information | |
Cat. No. | E01K0079 |
Product Name | Human Kallikrein 5 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
| KLK5 ELISA kit uses an anti-KLK5 antibody and an KLK5-HRP conjugate in a competitive enzyme immunoassay method. KLK5-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-KLK5 antibody binding site between KLK5 from samples and KLK5-HRP conjugate, the intensity of the color is inversely proportional to the concentration of KLK5. Since the number of sites is limited, as more sites are occupied by KLK5 from the sample, fewer sites are left to bind KLK5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLK5 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between KLK5 and analogues was observed. | |
E01K0079 has been referenced in the below publications：
KLK-L2, KLKL2, SCTE, Kallikrein-Related Peptidase 5, Kallikrein-like protein 2, Stratum corneum tryptic enzyme.
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