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BlueGene Biotech Human Thyroxine Binding Globulin ELISA kit (E01T0095)

E01T0095 Human Thyroxine Binding Globulin ELISA kit

The Human Thyroxine Binding Globulin ELISA kit can be used to identify samples from the human species. Thyroxine Binding Globulin can also be called SERPINA7, TBG, serpin family A member 7, Thyroxine-binding globulin,TBG, TBGQTL.

Products

Specifications of Human Thyroxine Binding Globulin ELISA kit

Product Information

Cat. No.

E01T0095

Product Name

Human Thyroxine Binding Globulin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

10-250 ng/ml

Sensitivity

1.0 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TBG ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TBG antibody and an TBG-HRP conjugate. The assay sample and buffer are incubated together with TBG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TBG concentration since TBG from samples and TBG-HRP conjugate compete for the anti-TBG antibody binding site. Since the number of sites is limited, as more sites are occupied by TBG from the sample, fewer sites are left to bind TBG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TBG concentration in each sample is interpolated from this standard curve. 


Quality Control on Human Thyroxine Binding Globulin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TBG and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Thyroxine Binding Globulin ELISA kit

Summary of the Assay Procedure for Human Thyroxine Binding Globulin ELISA kit

Citations of Human Thyroxine Binding Globulin ELISA kit

E01T0095 has been referenced in the below publications:

Potential urine proteomics biomarkers for primary nephrotic syndrome.

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