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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Survivin ELISA kit

E01S0218 Human Survivin ELISA kit

The Human Survivin ELISA kit can be used to identify samples from the human species. Survivin can also be called Surv, BIRC5, API4, EPR-1, baculoviral IAP repeat containing 5.


Specifications of Human Survivin ELISA kit

Product Information

Cat. No.

E01S0218

Product Name

Human Survivin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Surv ELISA kit uses an anti-Surv antibody and an Surv-HRP conjugate in a competitive enzyme immunoassay method. Surv-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Surv antibody binding site between Surv from samples and Surv-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Surv. Since the number of sites is limited, as more sites are occupied by Surv from the sample, fewer sites are left to bind Surv-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Surv concentration in each sample is interpolated from this standard curve.


Quality Control on Human Survivin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Surv and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Survivin ELISA kit

Summary of the Assay Procedure for Human Survivin ELISA kit

Citations of Human Survivin ELISA kit

E01S0218 has been referenced in the below publications:

Effects of Gonadotropins on the Expression of VEGF and Survivin in the Mouse Ovaries and the Birth Rate after Vitrification Cryopreserved Transplantation.

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