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BlueGene Biotech Human Immunoglobulin 2Alpha ELISA kit (E01I0060)

E01I0060 Human Immunoglobulin 2Alpha ELISA kit

The Human Immunoglobulin 2Alpha ELISA kit can be used to identify samples from the human species. Immunoglobulin 2Alpha can also be called IgG2Alpha, IgG2a.

Products

Specifications of Human Immunoglobulin 2Alpha ELISA kit

Product Information

Cat. No.

E01I0060

Product Name

Human Immunoglobulin 2Alpha ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ug/ml

Sensitivity

1.0 ug/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

50 ug/mL

1 vial

STANDARD C (0.5mL)

100 ug/mL

1 vial

STANDARD D (0.5mL)

250 ug/mL

1 vial

STANDARD E (0.5mL)

500 ug/mL

1 vial

STANDARD F (0.5mL)

1000 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IgG2Alpha ELISA kit uses an anti-IgG2Alpha antibody and an IgG2Alpha-HRP conjugate in a competitive enzyme immunoassay method. IgG2Alpha-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IgG2Alpha antibody binding site between IgG2Alpha from samples and IgG2Alpha-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IgG2Alpha. Since the number of sites is limited, as more sites are occupied by IgG2Alpha from the sample, fewer sites are left to bind IgG2Alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgG2Alpha concentration in each sample is interpolated from this standard curve.


Quality Control on Human Immunoglobulin 2Alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IgG2Alpha and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Immunoglobulin 2Alpha ELISA kit

Summary of the Assay Procedure for Human Immunoglobulin 2Alpha ELISA kit

Citations of Human Immunoglobulin 2Alpha ELISA kit

E01I0060 has been referenced in the below publications:

Differential effects of formaldehyde exposure on airway inflammation and bronchial hyperresponsiveness in BALB/c and C57BL/6 mice.

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