E01Z0012 Human Zinc finger protein PLAG1 ELISA kit
The Human Zinc finger protein PLAG1 ELISA kit can be used to identify samples from the human species. Zinc finger protein PLAG1 can also be called PLAG1, PSA, SGPA, ZNF912, PLAG1 zinc finger, SRS4.
Specifications of Human Zinc finger protein PLAG1 ELISA kit
Product Information | |
Cat. No. | E01Z0012 |
Product Name | Human Zinc finger protein PLAG1 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PLAG1 ELISA kit uses an anti-PLAG1 antibody and an PLAG1-HRP conjugate in a competitive enzyme immunoassay method. PLAG1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PLAG1 antibody binding site between PLAG1 from samples and PLAG1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PLAG1. Since the number of sites is limited, as more sites are occupied by PLAG1 from the sample, fewer sites are left to bind PLAG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLAG1 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Zinc finger protein PLAG1 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PLAG1 and analogues was observed. | |
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Summary of the Assay Procedure for Human Zinc finger protein PLAG1 ELISA kit
Citations of Human Zinc finger protein PLAG1 ELISA kit
E01Z0012 has been referenced in the below publications：
The expression level and clinical significane of IGF-1R、IGF-2、PLAG-1 in salivary pleomorphic adenoma in serum.
