Fish cyclic Adenosine Monophosphate ELISA kit is suitable for the detection of samples from fish species.
Specifications Of E17C0027 Fish cAMP ELISA Kit
Porduct's Information | |
Cat.NO. | E17C0027 |
Porduct's Name | Fish cyclic Adenosine Monophosphate ELISA kit |
Species | Fish |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pmol/ml |
Sensitivity | 1.0 pmol/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pmol/ml | 1 vial |
STANDARD B (0.5mL) | 50 pmol/ml | 1 vial |
STANDARD C (0.5mL) | 100 pmol/ml | 1 vial |
STANDARD D (0.5mL) | 250 pmol/ml | 1 vial |
STANDARD E (0.5mL) | 500 pmol/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pmol/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
cAMP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-cAMP antibody and an cAMP-HRP conjugate. The assay sample and buffer are incubated together with cAMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the cAMP concentration since cAMP from samples and cAMP-HRP conjugate compete for the anti-cAMP antibody binding site. Since the number of sites is limited, as more sites are occupied by cAMP from the sample, fewer sites are left to bind cAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cAMP concentration in each sample is interpolated from this standard curve. |
Quality Control On Fish cAMP ELISA Kit
CV | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery% | 85-113.86 | |
Linearity | Diluent ratio | Range % |
1:2 | 93-112 | |
1:4 | 91-108 | |
1:8 | 83-102 | |
Specificity/ Cross-reactivity | No significant cross-reactivity or interference between cAMP and analogues was observed. | |
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Summary Of The Assay Procedures For
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