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E17C0027 Fish Cyclic Adenosine Monophosphate ELISA Kit

Fish cyclic Adenosine Monophosphate ELISA kit is suitable for the detection of samples from fish species.

Specifications Of E17C0027 Fish cAMP ELISA Kit

Porduct's Information

Cat.NO.

E17C0027

Porduct's Name

Fish cyclic Adenosine Monophosphate ELISA kit

Species

Fish

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pmol/ml

Sensitivity

1.0 pmol/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pmol/ml

1 vial

STANDARD B (0.5mL)

50 pmol/ml

1 vial

STANDARD C (0.5mL)

100 pmol/ml

1 vial

STANDARD D (0.5mL)

250 pmol/ml

1 vial

STANDARD E (0.5mL)

500 pmol/ml

1 vial

STANDARD F (0.5mL)

1000 pmol/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial

Principle of the Assay

cAMP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-cAMP antibody and an cAMP-HRP conjugate. The assay sample and buffer are incubated together with cAMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the cAMP concentration since cAMP from samples and cAMP-HRP conjugate compete for the anti-cAMP antibody binding site. Since the number of sites is limited, as more sites are occupied by cAMP from the sample, fewer sites are left to bind cAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cAMP concentration in each sample is interpolated from this standard curve.


Quality Control On Fish cAMP ELISA Kit

CV

Intra Variation% <10%

Inter Variation% <12%

Recovery%

85-113.86

Linearity

Diluent ratio

Range %

1:2

93-112

1:4

91-108

1:8

83-102

Specificity/

Cross-reactivity

No significant cross-reactivity or interference between cAMP and analogues was observed.


BlueGene Biotech Product Show

Summary Of The Assay Procedures For

Summary Of The Assay Procedures For

Citations Of Fish Cyclic Adenosine Monophosphate ELISA Kit

Developmental toxicity by thifluzamide in zebrafish (Danio rerio): Involvement of leptin

Product: E17P0666 Fish Pyruvate Kinase A ELISA kit; E17L0038 Fish Leptin ELISA kit

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