Name: Fish Cyclic Adenosine Monophosphate ELISA kit
Brand: Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Fish Cyclic Adenosine Monophosphate ELISA kit

E17C0027 Fish Cyclic Adenosine Monophosphate ELISA kit

Fish cyclic Adenosine Monophosphate ELISA kit is suitable for the detection of samples from fish species. Cyclic Adenosine Monophosphate can also be called cAMP, AMP or 3',5'-cyclic adenosine monophosphate.

Products

Specifications of Fish Cyclic Adenosine Monophosphate ELISA kit

Product Information
Cat. No.	E17C0027
Product Name	Fish cyclic Adenosine Monophosphate ELISA kit
Species	Fish
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pmol/ml
Sensitivity	1.0 pmol/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pmol/ml	1 vial
STANDARD B (0.5mL)	50 pmol/ml	1 vial
STANDARD C (0.5mL)	100 pmol/ml	1 vial
STANDARD D (0.5mL)	250 pmol/ml	1 vial
STANDARD E (0.5mL)	500 pmol/ml	1 vial
STANDARD F (0.5mL)	1000 pmol/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

cAMP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-cAMP antibody and an cAMP-HRP conjugate. The assay sample and buffer are incubated together with cAMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the cAMP concentration since cAMP from samples and cAMP-HRP conjugate compete for the anti-cAMP antibody binding site. Since the number of sites is limited, as more sites are occupied by cAMP from the sample, fewer sites are left to bind cAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cAMP concentration in each sample is interpolated from this standard curve.

Quality Control on Fish Cyclic Adenosine Monophosphate ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	85-113.86%
Linearity	Diluent Ratio	Range %
	1:2	93-112
	1:4	91-108
	1:8	83-102
Specificity/Cross-reactivity	No significant cross-reactivity or interference between cAMP and analogues was observed.