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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Fish Testosterone ELISA kit (E17T0007)

  • Signal Transduction
  • Developmental Biology

E17T0007 Fish Testosterone ELISA kit

Fish Testosterone ELISA kit can be used to identify samples from the bovine species. Testosterone can also be called Testesteron, Testeronun, TESTO.

Products

Specifications of Fish Testosterone ELISA kit

Product Information

Cat. No.

E17T0007

Product Name

Fish Testosterone ELISA kit

Species

Fish

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TESTO ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TESTO. Standards or samples are then added to the microtiter plate wells and TESTO if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TESTO present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TESTO are added to each well to “sandwich” the TESTO immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TESTO and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TESTO concentration in each sample is interpolated from this standard curve


Quality Control on Fish Testosterone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TESTO and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Fish Testosterone ELISA kit

Summary of the Assay Procedure for Fish Testosterone ELISA kit

Citations of Fish Testosterone ELISA kit

E17T0007 has been referenced in the below publications:

The Kiss2/GPR54 system stimulates the reproductive axis in male black porgy, Acanthopagrus schlegelii.



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