High Five (H5) Host Cell Protein OneStep ELISA Kit, G3 is for research use only; it should not be used in clinical diagnostic procedures.
This kit is intended for the quantitative Measurement of High Five (H5) HCP Residues in Protein Purification Process, and End-Product.
| Product's Information | |
| Cat. NO. | HF-H0026-3D1-48T |
| Products Name | High Five (H5) Host Cell Protein OneStep ELISA Kit, G3 |
| Species | High Five |
| Product Size | 48 Tests |
| Concentration | 12.5-800ng/mL |
| Sensitivity | LOD: 3.125 ng/mL; LOQ: 12.5 ng/mL |
| Principal | Sandwich ELISA |
| Sample Volume | 100 ul |
| Sample Type | This kit is intended for the quantitative Measurement of HCP Residues in Protein Purification Process, and End-Product. |
| Assay Time | 2 hours and 15 minutes |
| Platform | Microplate Reader |
| Conjugate | HRP |
| Detection Method | Colorimetric |
| Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 48 wells | 1 plate (Keep Sealed) |
Standard (Stock Solution) -500ug/ml | 30ul | 1 tube |
Detection antibody Stock Solution(100×) | 100ul | 1 tube |
Substrates | 10ml | 1 vial (Avoid Light) |
Stop Solution | 10ml | 1 vial |
Wash Solution (20×) | 10ml | 5 vial |
Diluent Buffer (10×) | 10ml | 1 vial |
Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
Principle of the Assay |
This ELISA kit adopts the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with capture antibodies to prepare solid-phase antibodies. The sample antigen and the detection antibodies are added simultaneously to the microplate wells, forming a capture antibody-antigen-detection antibody complex. Wash the plate to remove unbound conjugates, then add substrate for color development. Finally, add stop solution to terminate the reaction. The color of the substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 405nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by inputting the OD values of the samples into the standard curve equation, calculate the concentration of HCP in the sample. |
CV | Intra Variation%: <10% | ||
Inter Variation%:<10% | |||
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