En
Email
  • host cell dna residue detection kit
  • host cell dna residue detection kit

BlueGene Biotech Pichia Pastoris Host Cell DNA Residue Detection Kit-100T

PP-D100T Pichia Pastoris Host Cell DNA Residue Detection kit

The Pichia Pastoris Host Cell DNA Residue Detection Kit can be used for Quantitative analysis of DNA contaminants in recombinant protein expression, intermediate purification, and finished production from host cells. 

Specifications of Pichia Pastoris Host Cell DNA Residue Detection kit

Introduction

This kit adopts Taqman probe fluorescence qPCR method. The kit has the advantages of high specificity and sensitivity by using specific primers & probes, LOD can reach 1fg level.The preparation process of DNA Control is completely consistent with National Standard, therefore it has high purity and no protein and ion interference to ensure the accuracy of the sample quantitative detection.The kit provides DNA Dilution Buffer, which enables good replicate parallelism in a single experiment and good reproducibility between multiple experiments.


Kit Components

DNA Amplification

Components NO.

Components Name

Cat#/Size PP-D100T(100T)

B1

2XqPCR Mix

1.25mL

B2

Primer&Probe Mix

200μL

B3

DNA Dilution Buffer

4×1.5mL

B4

DNA Control (10ng/μL)

50μL

B5

RNase-Free H2O

1mL

B6

50X ROX Reference Dye(Optional)

0.3ml


Shipping and Storage
1All components are shipped on dry ice.
2The kit should be stored at -20°C and has a shelf life of two years. B2 should be stored protected from light.


Quality Control on Pichia Pastoris Host Cell DNA Residue Detection kit

Accuracy (Standard: ≦30%)

Intra Variation% 3.1-10 

Inter Variation% 10-24

Recovery

69-127%

Limit of Quantitation10fg/μL

Specificity

-




BlueGene Biotech Product Show

PCR Reaction System on Pichia Pastoris Host Cell DNA Residue Detection kit


Components

Volume(μL)             

2XqPCR Mix

12.5

Primer&Probe Mix

2

DNA template (control or sample)

5

Add water

5.5

Total Volume

25


  • Mix solution = (number of reaction wells+4) * (12.5+2+5.5)μL (including the volume lost in the 4 wells).


  • The detection range of the standard curve mentioned above is suitable for most experiments and can be adjusted as needed, such as 3fg/ul-300pg/ul.

Criteria for Results

  • Standard Curve: R ²> 0.99; Amplification Efficiency: 90% ≤ Eff% ≤ 110%; Slope: -3.8~-3.1.


  • The recovery rate of spiked samples=(measured value of spiked samples - measured value of samples)/theoretical value of spiked samples * 100%, with a range of 50% -150%.


  • Negative Quality Control (NC): The reaction system contains only DNA Dilution Buffer, and the detection result should have a Ct value greater than the mean Ct value of the lowest concentration Ct on the standard curve.


  • No Template Control (NTC): Replace only the DNA template with DNA Dilution Buffer in the reaction system, and the Ct value obtained should be 'Undetermined' or ≥35.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.