E02C0549 Rat Caspase 3 ELISA kit
The Rat Caspase 3 ELISA kit can be used to identify samples from the rat species. Caspase 3 can also be called CPP32, CPP32B, SCA-1, caspase 3, CASP3.
Specifications of Rat Caspase 3 ELISA kit
Product Information | |
Cat. No. | E02C0549 |
Product Name | Rat Caspase 3 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD E (0.5mL) | 10 ng/ml | 1 vial |
STANDARD F (0.5mL) | 25 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CASP3 ELISA kit uses an anti-CASP3 antibody and an CASP3-HRP conjugate in a competitive enzyme immunoassay method. CASP3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CASP3 antibody binding site between CASP3 from samples and CASP3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CASP3. Since the number of sites is limited, as more sites are occupied by CASP3 from the sample, fewer sites are left to bind CASP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CASP3 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Caspase 3 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CASP3 and analogues was observed. | |
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Summary of the Assay Procedure for Rat Caspase 3 ELISA kit
Citations of Rat Caspase 3 ELISA kit
E02C0549 has been referenced in the below publications：
The Study on Selection of Excellent Bacillus subtilis and Determining its' Growth Performance and Some Biological Indexes.
化痰方对非酒精性脂肪性肝炎细胞因子和Caspase-3含量的影响※。
Effect of Buyang Huanwu Decoction on Neuron Apoptosis and Gene Expression of Caspase-3 in Brains of Rats after Cerebral Ischemia Reperfusion Injury.
The interference of picrosideⅡ on the expressions of Caspase3 and PARP following cerebral ischemia reperfusion injuryin rats.
Comparative study of cardio-protective effects of zinc oxide nanoparticles and zinc sulfate in streptozotocin-induced diabetic rats.
