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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Active Caspase 3 ELISA kit (E02A0015)

E02A0015 Rat Active Caspase 3 ELISA kit

Rat Active caspase 3 ELISA kit is suitable for the detection of samples from Rat species. Active caspase 3 can also be called CPP32, CPP32B, SCA1, Apoptain, Yama, Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 3, SREBP cleavage activity 1, Active casp3, casp3, casp-3, casp 3.

Products

Specifications of Rat Active caspase 3 ELISA kit

Product Information

Cat. No.

E02A0015

Product Name

Rat Active caspase 3 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5.0-100ng/ml

Sensitivity

1.0 ng/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C



Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ACTIVE CASPASE 3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ACTIVE CASPASE 3. Standards or samples are then added to the microtiter plate wells and ACTIVE CASPASE 3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ACTIVE CASPASE 3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ACTIVE CASPASE 3 are added to each well to “sandwich” the ACTIVE CASPASE 3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ACTIVE CASPASE 3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTIVE CASPASE 3 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Active caspase 3 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-108%

Linearity

Diluent Ratio

Range %

1:2

95-105

1:4

93-104

1:8

89-109

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Active CASP3 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Active caspase 3 ELISA kit

Summary of the Assay Procedure for Rat Active caspase 3 ELISA kit

Citations of Rat Active caspase 3 ELISA kit

E02A0015 has been referenced in the below publications:

Hypoxic Preconditioning Augments the Therapeutic Efficacy of Bone Marrow Stromal Cells in a Rat Ischemic Stroke Model.

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