E02C0348 Rat Carcinoembryonic Antigen ELISA kit
The Rat Carcinoembryonic Antigen ELISA kit can be used to identify samples from the rat species. Carcinoembryonic Antigen can also be called CD66E; CD66; CEACAM5; Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; Carcinoembryonic Antigen, CEA.
Product Information | |
Cat. No. | E02C0348 |
Product Name | Rat Carcinoembryonic Antigen ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 10 ng/ml | 1 vial |
STANDARD C (0.5mL) | 25 ng/ml | 1 vial |
STANDARD D (0.5mL) | 50 ng/ml | 1 vial |
STANDARD E (0.5mL) | 100 ng/ml | 1 vial |
STANDARD F (0.5mL) | 250 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CEA ELISA kit uses an anti-CEA antibody and an CEA-HRP conjugate in a competitive enzyme immunoassay method. CEA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CEA antibody binding site between CEA from samples and CEA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CEA. Since the number of sites is limited, as more sites are occupied by CEA from the sample, fewer sites are left to bind CEA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CEA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CEA and analogues was observed. | |
E02C0348 has been referenced in the below publications：
The Effects of Radon and Its Daughter on Targeted Organs and Protective Effect of Radon-protection agent in Rats.
Effects of radon on the gene methylation in BALF cells and tumor markers in serum with rats as experimental mode.
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