E02I0345 Rat Interferon γ ELISA kit
The Rat Interferon γ ELISA kit can be used to identify samples from the rat species. Interferon γ can also be called IFNγ, IFG, IFI, IFN Gamma, IFNG.
Specifications of Rat Interferon γ ELISA kit
Product Information | |
Cat. No. | E02I0345 |
Product Name | Rat Interferon γ ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IFNγ ELISA kit uses an anti-IFNγ antibody and an IFNγ-HRP conjugate in a competitive enzyme immunoassay method. IFNγ-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IFNγ antibody binding site between IFNγ from samples and IFNγ-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IFNγ. Since the number of sites is limited, as more sites are occupied by IFNγ from the sample, fewer sites are left to bind IFNγ-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNγ concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Interferon γ ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IFNγ and analogues was observed. | |
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Summary of the Assay Procedure for Rat Interferon γ ELISA kit
Citations of Rat Interferon γ ELISA kit
E02I0345 has been referenced in the below publications：
Effects of colon targeting microecological modulator nano CYP synbiotics on cytokines and expression of SOD,MDA, NO and MPO in dysbacteria wistar rats.
Intrahepatic transplantation of adipose‑derived stem cells attenuates the progression of non‑alcoholic fatty liver disease in rats.
Neuroprotective effect of (‑)‑epigallocatechin‑3‑gallate on autoimmune thyroiditis in a rat model by an anti‑inflammation effect, anti‑apoptosis and inhibition of TRAIL signaling pathway.
