Rat Cytochrome P450 7A1 ELISA kit - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Rat Cytochrome P450 7A1 ELISA kit

E02C0375 Rat Cytochrome P450 7A1 ELISA kit

The Rat Cytochrome P450 7A1 ELISA kit can be used to identify samples from the rat species. Cytochrome P450 7A1 can also be called CYP7A1, CP7A, CYP7, CYPVII, cytochrome P450 family 7 subfamily A member 1.

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Specifications of Rat Cytochrome P450 7A1 ELISA kit

Product Information
Cat. No.	E02C0375
Product Name	Rat Cytochrome P450 7A1 ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	100-2500 pg/ml
Sensitivity	1.0 pg/ml
Principal	Sandwich ELISA
Sample Volume	50 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	10 mL	1 vial
STANDARD A (0.5mL)	0 pg/ml	1 vial
STANDARD B (0.5mL)	100 pg/ml	1 vial
STANDARD C (0.5mL)	250 pg/ml	1 vial
STANDARD D (0.5mL)	500 pg/ml	1 vial
STANDARD E (0.5mL)	1000 pg/ml	1 vial
STANDARD F (0.5mL)	2500 pg/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

CYP7A1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CYP7A1. Standards or samples are then added to the microtiter plate wells and CYP7A1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CYP7A1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CYP7A1 are added to each well to “sandwich” the CYP7A1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CYP7A1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP7A1 concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Cytochrome P450 7A1 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between CYP7A1 and analogues was observed.