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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Glucagon Like Peptide 1 ELISA kit (E02G0031)

E02G0031 Rat Glucagon Like Peptide 1 ELISA kit

The Rat Glucagon Like Peptide 1 ELISA kit can be used to identify samples from the rat species. Glucagon Like Peptide 1 can also be called GCG, Glicentin related polypeptide, GLP 2, Glucagon like peptide 1, Glucagon like peptide 2, Glucagon precursor, GRPP.

Products

Specifications of Rat Glucagon Like Peptide 1 ELISA kit

Product Information

Cat. No.

E02G0031

Product Name

Rat Glucagon Like Peptide 1 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/mL

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GLP 1 ELISA kit uses an anti-GLP 1 antibody and an GLP 1-HRP conjugate in a competitive enzyme immunoassay method. GLP 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GLP 1 antibody binding site between GLP 1 from samples and GLP 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GLP 1. Since the number of sites is limited, as more sites are occupied by GLP 1 from the sample, fewer sites are left to bind GLP 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLP 1 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Glucagon Like Peptide 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GLP 1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Glucagon Like Peptide 1 ELISA kit

Summary of the Assay Procedure for Rat Glucagon Like Peptide 1 ELISA kit

Citations of Rat Glucagon Like Peptide 1 ELISA kit

E02G0031 has been referenced in the below publications:

Effect of Sijunzitang on the Level of GLP-1 and Islet Function in Spleen Diabetic Rats.

低频电针“足三里”T2DM大鼠GLP-1分泌影响的实验研究。

Influence of Bariatric Surgery on glucose metabolism of normal rats .

Effect of Liraglutide on improving the glucose metabolism by inhibiting toll-like recpter 4/ toll-like recpter 4 signaling pathway in type 2 diabetes rats.

Clinical Observation and Mechanism of Spleen Qi Treatment of Spleen Deficiency Type 2 Diabetes.

Effects of aerobic exercising combined with glutamine supplement on the serum GLP-1, insulin and blood glucose levels of rats with T2DM.


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