En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Inhibin ELISA kit

E02I0012 Rat Inhibin ELISA kit

The Rat Inhibin ELISA kit can be used to identify samples from the rat species. Inhibin can also be called INH.

Specifications of Rat Inhibin ELISA kit

Product Information

Cat. No.

E02I0012

Product Name

Rat Inhibin ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

INH ELISA kit uses an anti-INH antibody and an INH-HRP conjugate in a competitive enzyme immunoassay method. INH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-INH antibody binding site between INH from samples and INH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of INH. Since the number of sites is limited, as more sites are occupied by INH from the sample, fewer sites are left to bind INH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INH concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Inhibin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between INH and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Inhibin ELISA kit0518

Summary of the Assay Procedure for Rat Inhibin ELISA kit0518

Citations of Rat Inhibin ELISA kit

E02I0012 has been referenced in the below publications:

In vivo transplantation of 3D encapsulated ovarian constructs in rats corrects abnormalities of ovarian failure.


Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.