NE11I0431 Bovine Immunoglobulin G (IgG) ELISA kit
This kit is for the quantitative Measurement of bovine IgG Residues in Protein Purification Process, and End-Product (purified fermentation broth, cell culture supernatant, etc.)
Specifications of Bovine Immunoglobulin G (IgG) ELISA kit
Product Information | |
Cat. NO. | NE11I0431 |
Product Name | Bovine Immunoglobulin G (IgG) ELISA kit |
Species | Bovine |
Product Size | 96 Tests |
Concentration | 78.125-5000 pg/ml |
Sensitivity | 26.8 pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 100 ul |
Sample Type | ELISA Kit for the quantitative Measurement of bovine IgG Residues in Protein Purification Process, and End-Product (purified fermentation broth, cell culture supernatant, etc.) |
Assay Time | 3 hours and 15 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 96 wells | 1 plate (Keep Sealed) |
| Standard (Stock Solution) -5ug/ml | 10ul | 1 vial |
| HRP-conjugated antibody(100×) | 150ul | 1 vial |
Diluent Buffer (10×) | 10ml | 1 vial |
TMB Substrates | 10ml | 1 vial (Avoid Light) |
Stop Solution | 10ml | 1 vial |
Wash Solution (100×) | 10ml | 1 vial |
Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
Principle of the Assay |
This ELISA kit is applied to the double antibody sandwich Enzyme Linked Immunosorbent Assay to detect the concentration of bovine IgG in samples. The microtiter plate has been pre-coated with rabbit anti-bovine antibody , standards or samples are then added to the microtiter plate wells and IgG if present, will bind to the antibody pre-coated wells under specific incubation conditions. Then wash the plate to remove any unbound substances. Then add HRP-conjugated rabbit anti-bovine antibody and incubate at room temperature to form a precoated antibody-bovine IgG-detection antibody sandwich complex. After washing the plate, add the TMB substrate solution to the wells. Upon completion of the color development, add Stop Solution to terminate the reaction, and the color will change from blue to yellow. Color of TMB substrate positively correlated with total IgG bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of bovine IgG in samples is then determined by comparing the O.D. of the samples to the standard curve. |
Quality Control on Bovine Immunoglobulin G (IgG) ELISA kit
Coefficient of Variance | Intra Variation: 0-4% | |
Inter Variation: 5-13% | ||
Recovery | 80-120% | |
Linearity | Diluent Ratio | Range % |
1: 2 | 87.54-100.00 | |
1: 4 | 100.10-103.98 | |
1: 8 | 98.75-107.51 | |
1: 16 | 101.64-105.10 | |
1: 32 | 100.80-102.80 | |
Specificity/ Cross-reactivity | Sample | Cross-Reactivity（%） |
Mouse IgG | 0.033 | |
Goat IgG | 0.266 | |
Human IgG | 0.019 | |
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Summary of the Assay Procedure for Bovine Immunoglobulin G (IgG) ELISA kit VDR
Citations of Bovine Immunoglobulin G (IgG) ELISA kit
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