Rat Albumin ELISA kit is suitable for the detection of samples from Rat species. Albumin can also be called as ALB; Serum albumin.
Specifications of E02A0028 Rat ALB ELISA Kit
Porduct's Information | |
Cat.NO. | E02A0028 |
Porduct's Name | Rat Albumin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ALB ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ALB. Standards or samples are then added to the microtiter plate wells and ALB if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ALB present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ALB are added to each well to “sandwich” the ALB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ALB and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALB concentration in each sample is interpolated from this standard curve. |
Quality Control On Rat ALB ELISA Kit
CV | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery% | 92-110 | |
Linearity | Diluent ratio | Range % |
1:2 | 94-102 | |
1:4 | 92-105 | |
1:8 | 89-113 | |
Specificity/ Cross-reactivity | No significant cross-reactivity or interference between ALB and analogues was observed. | |
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Summary of The Assay Procedures For E02A0028 Rat ALB ELISA Kit
Citations of Rat Albumin ELISA Kit
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