E02P0012 Rat Prostaglandin E2 ELISA kit
The Rat Prostaglandin E2 ELISA kit can be used to identify samples from the rat species. Prostaglandin E2 can also be called EP 2, EP2, Prostaglandin E Receptor EP2, PGE receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostaglandin E receptor 2 EP2 subtype, Prostaglandin E receptor 2 subtype EP2 53kDa, Prostaglandin E receptor 2 subtype EP2, Prostaglandin E2 receptor, Prostaglandin E2 receptor EP2 subtype, Prostanoid EP2 receptor, PTGER 2, PTGER2.
Product Information | |
Cat. No. | E02P0012 |
Product Name | Rat Prostaglandin E2 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PGE2 ELISA kit uses an anti-PGE2 antibody and an PGE2-HRP conjugate in a competitive enzyme immunoassay method. PGE2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PGE2 antibody binding site between PGE2 from samples and PGE2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PGE2. Since the number of sites is limited, as more sites are occupied by PGE2 from the sample, fewer sites are left to bind PGE2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGE2 concentration in each sample is interpolated from this standard curve |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PGE2 and analogues was observed. | |
E02P0012 has been referenced in the below publications：
Mechanisms of Anti-inflammatory Property of Conserved Dopamine Neurotrophic Factor: Inhibition of JNK Signaling in Lipopolysaccharide-Induced Microglia.
Guijia Shutong Capsule in the treatment of primary Experimental study on smenorrhea.
Experimental Study on Therapeutic Effect of Sanqi HuoXue Tablet on Acute Soft Tissue Injury of Rats.
Impacts of abdominal vibration tuina therapy on primary dysmenorrheal in the rat models.
祖师麻肠溶制剂的药效学研究。
An experimental model of rat reflux gastritis based on metabolomics.
Renoprotective effect of paricalcitol in rats with diabetic nephropathy.
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