E02L0018 Rat Laminin ELISA kit
The Rat Laminin ELISA kit can be used to identify samples from the rat species. Laminin can also be called LAM, LN.
Specifications of Rat Laminin ELISA kit
Product Information | |
Cat. No. | E02L0018 |
Product Name | Rat Laminin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LN ELISA kit uses an anti-ILN antibody and an ILN-HRP conjugate in a competitive enzyme immunoassay method. ILN-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ILN antibody binding site between ILN from samples and ILN-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ILN. Since the number of sites is limited, as more sites are occupied by ILN from the sample, fewer sites are left to bind ILN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ILN concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Laminin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LN and analogues was observed. | |
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Summary of the Assay Procedure for Rat Laminin ELISA kit
Citations of Rat Laminin ELISA kit
E02L0018 has been referenced in the below publications：
Studies on functional effect and mechanisms of anti-alcohol and liver-protection of Lingzhisuan Granule.
Protective effects and mechanism of matrine on high-fructose-diet induced non-alcoholic fatty liver in rats.
Comparison of hepatic fibrosis models and associated hepatic fibronectin expression in Wistar rats treated by bile duct ligation and CCl4.
