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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Secretoglobin, Family 1A, Member 1 ELISA Kit (E02U0019)

E02U0019 Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit

The Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit can be used to identify samples from the rat species. Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 can also be called SCGB1A1, CC10, CC16, CCPBP, CCSP, UGB, UP-1, UP1, secretoglobin family 1A member 1.    

Products

Specifications of Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit

Product Information

Cat. No.

E02U0019

Product Name

Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SCGB1A1/CC16 ELISA kit uses an anti-SCGB1A1/CC16 antibody and an SCGB1A1/CC16-HRP conjugate in a competitive enzyme immunoassay method. SCGB1A1/CC16-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SCGB1A1/CC16 antibody binding site between SCGB1A1/CC16 from samples and SCGB1A1/CC16-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SCGB1A1/CC16. Since the number of sites is limited, as more sites are occupied by SCGB1A1/CC16 from the sample, fewer sites are left to bind SCGB1A1/CC16-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCGB1A1/CC16 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SCGB1A1/CC16 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit

Summary of the Assay Procedure for Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit

Citations of Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit

E02U0019 has been referenced in the below publications:

Analysis of Components in Bronchoalveolar Lavage Fluid of Rats following silica exposure.

Study on levels of silicon and CC16 in serum of rats exposd to silica dust.

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