E02U0019 Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit
The Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit can be used to identify samples from the rat species. Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 can also be called SCGB1A1, CC10, CC16, CCPBP, CCSP, UGB, UP-1, UP1, secretoglobin family 1A member 1.
Product Information | |
Cat. No. | E02U0019 |
Product Name | Rat Secretoglobin, Family 1A, Member 1/Clara cell Specific protein CC16 ELISA Kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SCGB1A1/CC16 ELISA kit uses an anti-SCGB1A1/CC16 antibody and an SCGB1A1/CC16-HRP conjugate in a competitive enzyme immunoassay method. SCGB1A1/CC16-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SCGB1A1/CC16 antibody binding site between SCGB1A1/CC16 from samples and SCGB1A1/CC16-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SCGB1A1/CC16. Since the number of sites is limited, as more sites are occupied by SCGB1A1/CC16 from the sample, fewer sites are left to bind SCGB1A1/CC16-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCGB1A1/CC16 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SCGB1A1/CC16 and analogues was observed. | |
E02U0019 has been referenced in the below publications：
Analysis of Components in Bronchoalveolar Lavage Fluid of Rats following silica exposure.
Study on levels of silicon and CC16 in serum of rats exposd to silica dust.
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