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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Matrix metalloproteinsae 1 ELISA kit

E02M0294 Rat Matrix metalloproteinsae 1 ELISA kit

The Rat Matrix metalloproteinsae 1 ELISA kit can be used to identify samples from the rat species. Matrix metalloproteinsae 1 can also be called CLGN, CLG1, Collagenase, Interstitial Collagenase, Vertebrate Collagenase, Fibroblast Collagenase, MMP 1.

Specifications of Rat Matrix metalloproteinsae 1 ELISA kit

Product Information

Cat. No.

E02M0294

Product Name

Rat Matrix metalloproteinsae 1 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MMP 1 ELISA kit uses an anti-MMP 1 antibody and an MMP 1-HRP conjugate in a competitive enzyme immunoassay method. MMP 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MMP 1 antibody binding site between MMP 1 from samples and MMP 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MMP 1. Since the number of sites is limited, as more sites are occupied by MMP 1 from the sample, fewer sites are left to bind MMP 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP 1 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Matrix metalloproteinsae 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MMP 1 and analogues was observed.


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Summary of the Assay Procedure for Rat Matrix metalloproteinsae 1 ELISA kit

Summary of the Assay Procedure for Rat Matrix metalloproteinsae 1 ELISA kit

Citations of Rat Matrix metalloproteinsae 1 ELISA kit

E02M0294 has been referenced in the below publications:

Effects of Single and Combined Losartan and Tempol Treatments on Oxidative Stress,Kidney Structure and Function in Spontaneously Hypertensive Rats with Early Course of Proteinuric Nephropathy.


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