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BlueGene Biotech Human Pulmonary Activation Regulated Chemokine ELISA kit (E01P0061)

E01P0061 Human Pulmonary Activation Regulated Chemokine ELISA kit

The Human Pulmonary Activation Regulated Chemokine ELISA kit can be used to identify samples from the human species. Pulmonary Activation Regulated Chemokine can also be called CCL18, AMAC1, SCYA18, DCCK1, MIP-4, CKb7, Alternative Macrophage Activation Associated CC Chemokine 1, Chemokine C-C-Motif Ligand 18, Macrophage inflammatory protein 4.

Products

Specifications of Human Pulmonary Activation Regulated Chemokine ELISA kit

Product Information

Cat. No.

E01P0061

Product Name

Human Pulmonary Activation Regulated Chemokine ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PARC ELISA kit uses an anti-PARC antibody and an PARC-HRP conjugate in a competitive enzyme immunoassay method. PARC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PARC antibody binding site between PARC from samples and PARC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PARC. Since the number of sites is limited, as more sites are occupied by PARC from the sample, fewer sites are left to bind PARC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PARC concentration in each sample is interpolated from this standard curve.


Quality Control on Human Pulmonary Activation Regulated Chemokine ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PARC and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Pulmonary Activation Regulated Chemokine ELISA kit

Summary of the Assay Procedure for Human Pulmonary Activation Regulated Chemokine ELISA kit

Citations of Human Pulmonary Activation Regulated Chemokine ELISA kit

E01P0061 has been referenced in the below publications:

The expression level and value of CCL-18 in the serum of the patients with Chronic Obstructive Pulmonary Disease.

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